Rabbit Anti-phospho-LIM Kinase 2 (Thr505) antibody

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LIM Kinase 1 (phospho T505); p-LIM Kinase 1 (phospho T505); LIM domain kinase 2; LIMK 2; LIMK-2; LIMK2; LIMK2_HUMAN.

Cat:

SL18257R

Species Reactivity：

(predicted: Human,Mouse,Rat,Chicken,Dog,Pig,Cow,Horse,Rabbit,Zebrafish,Sheep,)

Immunogen：

KLH conjugated synthesised phosphopeptide derived from human LIM Kinase 1 around the phosphorylation site of Thr505:RY(p-T)VV

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

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Unit:

Price: $256.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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There are approximately 40 known eukaryotic LIM proteins, so named for the LIM domains they contain. LIM domains are highly conserved cysteine-rich structures containing 2 zinc fingers. Although zinc fingers usually function by binding to DNA or RNA, the LIM motif probably mediates protein-protein interactions. LIM kinase-1 and LIM kinase-2 belong to a small subfamily with a unique combination of 2 N-terminal LIM motifs and a SLCterminal protein kinase domain. The protein encoded by this gene is phosphorylated and activated by ROCK, a downstream effector of Rho, and the encoded protein, in turn, phosphorylates cofilin, inhibiting its actin-depolymerizing activity. It is thought that this pathway contributes to Rho-induced reorganization of the actin cytoskeleton. At least three transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Displays serine/threonine-specific phosphorylation of myelin basic protein and histone (MBP) in vitro.

Subcellular Location:
Cytoplasm. Nucleus. Isoform LIMK2a is distributed in the cytoplasm and the nucleus and Cytoplasm. Nucleus. Isoform LIMK2b occurs mainly in the cytoplasm and is scarcely translocated to the nucleus.

Tissue Specificity:
Highest expression in the placenta; moderate level in liver, lung, kidney, and pancreas. LIMK2a is found to be more abundant then LIMK2b in liver, colon, stomach, and spleen, while in brain, kidney, and placenta LIMK2b is the dominant form. In adult lung, both LIMK2a and LIMK2b is nearly equally observed.

Post-translational modifications:
Phosphorylated on serine and/or threonine residues by ROCK1.

Similarity:
Belongs to the protein kinase superfamily.
TKL Ser/Thr protein kinase family.
Contains 2 LIM zinc-binding domains.
Contains 1 PDZ (DHR) domain.
Contains 1 protein kinase domain.

SWISS:
P53671

Gene ID:
3985

Database links:

Entrez Gene: 3985 Human

Entrez Gene: 16886 Mouse

Entrez Gene: 29524 Rat

Omim: 601988 Human

SwissProt: P53671 Human

SwissProt: O54785 Mouse

SwissProt: P53670 Rat

Unigene: 474596 Human

Unigene: 124176 Mouse

Unigene: 390323 Mouse

Unigene: 442189 Mouse

Unigene: 11013 Rat

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