Rabbit Anti-MetAP1 antibody

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Methionyl Aminopeptidase 1; AMPM1_HUMAN; DKFZp781C0419; KIAA0094; MAP 1; MAP1A; MetAP-1; MetAP1; metap1; MetAP1A; Methionine aminopeptidase 1; Peptidase M 1.

Cat:

SL3766R

Species Reactivity：

Mouse,Rat,(predicted: Human,Chicken,Dog,Cow,Horse,Rabbit,)

Immunogen：

KLH conjugated synthetic peptide derived from human MetAP1:51-150/386

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

Product Overview：

Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MetAP1) Polyclonal Antibody, Unconjugated (SL3766R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MetAP1) Polyclonal Antibody, Unconjugated (SL3766R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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MetAP-1 is a 394-amino acid protein that is expressed at low levels in all tissues, but is highly expressed in skeletal muscles. The active site of MetAP-1 contains two adjacent divalent metal ions connected by a water molecule or hydroxide ion. The control of cell proliferation in mammalian cells is directly linked and strictly dependent on the evolutionarily highly conserved mechanism that MetAP-1 employs. Eukaryotes contain both MetAP-1 and MetAP-2, whereas prokaryotes possess only the MetAP-1 enzyme. Pyridine-2-carboxylic acid thiazol-2-ylamide (PCAT) forms a scaffold that inhibits the action of MetAP-1, while 1,2,4-triazol is a non-peptide inhibitor of MetAP-1 binding to the active site with the N1 and N2 atoms of the triazole moiety complexing two divalent ions.

Function:
Cotranslationally removes the N-terminal methionine from nascent proteins. The N-terminal methionine is often cleaved when the second residue in the primary sequence is small and uncharged (Met-Ala-, Cys, Gly, Pro, Ser, Thr, or Val). Required for normal progression through the cell cycle.

Subunit:
Associates with the 60S ribosomal subunit of the 80S translational complex.

Subcellular Location:
Cytoplasm.

Similarity:
Belongs to the peptidase M24A family.
Methionine aminopeptidase type 1 subfamily.

SWISS:
P53582

Gene ID:
23173

Database links:

Entrez Gene: 23173 Human

Entrez Gene: 75624 Mouse

Entrez Gene: 295500 Rat

Omim: 610151 Human

SwissProt: P53582 Human

SwissProt: Q8BP48 Mouse

Unigene: 96364 Human

Unigene: 26833 Mouse

Picture

Paraformaldehyde-fixed, paraffin embedded (mouse testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Antibody incubation with (MetAP1) Polyclonal Antibody, Unconjugated (SL3766R) at 1:200 overnight at 4°C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.

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