PRPK is a 253 amino acid protein kinase that phosphorylates Ser15 of p53. PRPK phosphorylation of p53 causes increased stabilization and activity of p53. CGI-121 may act as an inhibitor of the PRPK-p53 interaction, thus preventing the phosphorylation of p53. Unphosphorylated p53 is degraded by the ubiquitin-proteasome pathway, which may ultimately lead to cell proliferation. PRPK contains a protein kinase domain with a conserved catalytic core. PRPK is localized to the nucleus of the cell and is highly expressed in testis, with lower expression in heart, kidney and spleen.
Function:
Component of the EKC/KEOPS complex that is required for the formation of a threonylcarbamoyl group on adenosine at position 37 (t6A37) in tRNAs that read codons beginning with adenine. The complex is probably involved in the transfer of the threonylcarbamoyl moiety of threonylcarbamoyl-AMP (TSLCAMP) to the N6 group of A37. TP53RK has ATPase activity in the context of the EKC/KEOPS complex and likely plays a supporting role to the catalytic subunit OSGEP (By similarity). Atypical protein kinase that phosphorylates 'Ser-15' of p53/TP53 protein and may therefore participate in its activation.
Subcellular Location:
Nucleus.
Tissue Specificity:
Highly expressed in testis. Weakly expressed in heart kidney and spleen.
Similarity:
Belongs to the protein kinase superfamily.
Contains 1 protein kinase domain.
SWISS:
Q96S44
Gene ID:
112858
Database links:
Entrez Gene: 112858 Human
Entrez Gene: 76367 Mouse
Omim: 608679 Human
SwissProt: Q96S44 Human
SwissProt: Q99PW4 Mouse
Unigene: 88263 Human
Unigene: 330796 Mouse
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