The CCR4-NOT complex is an evolutionarily conserved, multi-component complex known to be involved in transcription as well as mRNA degradation. Various subunits within the complex are involved in influencing nuclear hormone receptor activities. The CCR4-NOT complex is also involved in the regulation of Histone H3 lysine 4 methylation through a ubiquitin-dependent pathway that likely involves the proteasome. CNOT4 (CCR4-NOT transcription complex subunit 4), also known as CCR4-associated factor 4 and E3 ubiquitin-protein ligase CNOT4, is a 575 amino acid protein that is a subunit of the CCR4-NOT complex. CNOT4 contains one C3H1-type zinc finger, one RING-type zinc finger and one RRM (RNA recognition motif) domain. Via its RING domain, CNOT4 binds E2 ubiquitin ligases. CNOT4 functions as a UbcH5B-dependent ubiquitin-protein ligase (E3 ligase). There are eight isoforms of CNOT4 that are expressed as a result of alternative splicing events.
Function:
Has E3 ubiquitin ligase activity. The CCR4-NOT complex functions as general transcription regulation complex.
Subunit:
Subunit of the CCR4-NOT core complex that contains CHAF1A, CHAF1B, CNOT1, CNOT2, CNOT3, CNOT4, CNOT6 and CNOT8. Binds CNOT1 via its SLCterminus. Binds E2 ubiquitin ligases via its RING domain. Interacts (via RING domain) with UBE2D2.
Subcellular Location:
Cytoplasm. Nucleus.
Post-translational modifications:
Autoubiquitinated.
Similarity:
Contains 1 C3H1-type zinc finger.
Contains 1 RING-type zinc finger.
Contains 1 RRM (RNA recognition motif) domain.
SWISS:
O95628
Gene ID:
4850
Database links:
Entrez Gene: 4850 Human
Entrez Gene: 53621 Mouse
Entrez Gene: 312227 Rat
Entrez Gene: 380304 Xenopus laevis
Omim: 604911 Human
SwissProt: O95628 Human
SwissProt: Q8BT14 Mouse
Unigene: 490224 Human
Unigene: 214525 Mouse
Unigene: 398182 Mouse
Unigene: 203387 Rat
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