Rabbit Anti-RASGRP2 antibody

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Calcium and DAG-regulated; Calcium and DAG-regulated guanine nucleotide exchange factor I; Calcium-and diacylglycerol-regulated guanine nucleotide exchange factor I; CalDAG-GEFI; Cdc25 like protein; Cdc25-like protein; F25B3.3 kinase like protein;

Cat:

SL19735R

Species Reactivity：

(predicted: Human,Mouse,Rat,Cow,)

Immunogen：

KLH conjugated synthetic peptide derived from human RASGRP2:351-450/609

Format：

Liquid

Storage instructions：

Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.

Concentration：

1mg/ml

Clonality：

Polyclonal

Isotype：

IgG

Applications：

WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500ICC=1:100-500IF=1:100-500（Paraffin sections need to do antigen repair）not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.

Host：

Rabbit

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Unit:

Price: $228.00

Product PDFs

Datasheet: Down Datasheet

Other Information
Citations
Protein Attributes
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The protein encoded by this gene is a brain-enriched nucleotide exchanged factor that contains an N-terminal GEF domain, 2 tandem repeats of EF-hand calcium-binding motifs, and a SLCterminal diacylglycerol/phorbol ester-binding domain. This protein can activate small GTPases, including RAS and RAP1/RAS3. The nucleotide exchange activity of this protein can be stimulated by calcium and diacylglycerol. Three alternatively spliced transcript variants encoding the same protein have been found for this gene. [provided by RefSeq, Jul 2008]

Function:
Functions as a calcium- and DAG-regulated nucleotide exchange factor specifically activating Rap through the exchange of bound GDP for GTP. May also activates other GTPases such as RRAS, RRAS2, NRAS, KRAS but not HRAS. Functions in aggregation of platelets and adhesion of T-lymphocytes and neutrophils probably through inside-out integrin activation. May function in the muscarinic acetylcholine receptor M1/CHRM1 signaling pathway.

Subcellular Location:
Cytoplasm > cytosol. Cell membrane. Cell junction > synapse > synaptosome. Cell projection > ruffle membrane. Found both in the cytosol and associated with membranes. Isoform 2 mainly localizes to the cell membrane. Enriched at juxtamembrane areas and membrane ruffles through association with F-actin. Localizes to the cell bodies and axons of striatal neurons.

Tissue Specificity:
Detected in platelets, neutrophils and T lymphocytes (at protein level). Expressed in brain where it is enriched in the striatum. Also expressed in the hematopoietic system. Detected in heart, brain, lung, placenta, liver, skeletal muscle and kidney.

Post-translational modifications:
Isoform 2 is palmitoylated and myristoylated while isoform 1.

Similarity:
Belongs to the RASGRP family.
Contains 2 EF-hand domains.
Contains 1 N-terminal Ras-GEF domain.
Contains 1 phorbol-ester/DAG-type zinc finger.
Contains 1 Ras-GEF domain.

SWISS:
Q7LDG7

Gene ID:
10235

Database links:

Entrez Gene: 10235 Human

Entrez Gene: 512056 Cow

Entrez Gene: 19395 Mouse

Entrez Gene: 361714 Rat

Omim: 605577 Human

SwissProt: A6N9I4 Cow

SwissProt: Q7LDG7 Human

SwissProt: Q9QUG9 Mouse

SwissProt: P0C643 Rat

Unigene: 99491 Human

Unigene: 77017 Mouse

Unigene: 38473 Rat

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