Home > Product > Antibody > Rabbit Anti-SLC25A41 antibody
S2541_HUMAN; Slc25a41; Solute carrier family 25 member 41.
Cat:
SL21237R
Species Reactivity:
Human,(predicted: Mouse,Cow,)
Immunogen:
KLH conjugated synthetic peptide derived from human SLC25A41:1-100/370
Format:
Liquid
Storage instructions:
Shipped at 4℃. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles.
Concentration:
1mg/ml
Clonality:
Polyclonal
Isotype:
IgG
Applications:
WB=1:500-2000ELISA=1:5000-10000IHC-P=1:100-500IHC-F=1:100-500Flow-Cyt=1ug/TestICC=1:100IF=1:100-500(Paraffin sections need to do antigen repair)not yet tested in other applications.optimal dilutions/concentrations should be determined by the end user.
Host:
Rabbit
Product Overview:
Blank control:THP-1. Primary Antibody (green line): Rabbit Anti-SLC25A41 antibody (SL21237R) Dilution: 1μg /10^6 cells; Isotype Control Antibody (orange line): Rabbit IgG . Secondary Antibody : Goat anti-rabbit IgG-FITCDilution: 1μg /test. ProtocolThe cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST methanol for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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Unit:
Price: $
Product PDFs
Datasheet:


SLC25A41 belongs to the SLC25 family of mitochondrial carrier proteins (Haitina et al., 2006 [PubMed 16949250]).[supplied by OMIM, Mar 2008]

Subcellular Location:
Mitochondrion inner membrane.

Similarity:
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats.

SWISS:
Q8N5S1

Gene ID:
284427

Database links:

Entrez Gene: 284427 Human

Omim: 610822 Human

SwissProt: Q8N5S1 Human

Unigene: 375135 Human



Picture

Blank control:THP-1.
Primary Antibody (green line): Rabbit Anti-SLC25A41 antibody (SL21237R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST methanol for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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