SLC25A41 belongs to the SLC25 family of mitochondrial carrier proteins (Haitina et al., 2006 [PubMed 16949250]).[supplied by OMIM, Mar 2008]
Subcellular Location:
Mitochondrion inner membrane.
Similarity:
Belongs to the mitochondrial carrier family.
Contains 3 Solcar repeats.
SWISS:
Q8N5S1
Gene ID:
284427
Database links:
Entrez Gene: 284427 Human
Omim: 610822 Human
SwissProt: Q8N5S1 Human
Unigene: 375135 Human
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Blank control:THP-1.
Primary Antibody (green line): Rabbit Anti-SLC25A41 antibody (SL21237R)
Dilution: 1μg /10^6 cells;
Isotype Control Antibody (orange line): Rabbit IgG .
Secondary Antibody : Goat anti-rabbit IgG-FITC
Dilution: 1μg /test.
Protocol
The cells were fixed with 4% PFA (10min at room temperature)and then permeabilized with 0.1% PBST methanol for 20 min at room temperature.The cells were then incubated in 5%BSA to block non-specific protein-protein interactions for 30 min at room temperature .Cells stained with Primary Antibody for 30 min at room temperature.The secondary antibody used for 40 min at room temperature. Acquisition of 20,000 events was performed.
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