Nitrate Reductase(NR) Activity Assay Kit

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硝酸还原酶(NR)活性检测试剂盒

Cat:

NA0865

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/24S

Storage instructions：

-20℃

Product Overview：

Components:

Inducer reserve fluid: Liquid 50 mL×1. Storage at 4℃. It should be prepared before using with 10 times

dilution. Prepare the reagent when it will be used.

Extraction reagent: Liquid 80 mL×1. Storage at 4℃.

Reagent I: Liquid 30 mL×1. Storage at -20℃.

Reagent II: Powder ×1. Storage at -20℃. Add 5 mL of Extraction reagent before using, mix thoroughly.

For long term preservation, separate into small tubules and storage at -20℃, avoid repeated freezing and

thawing. It can be stored for two weeks at -20℃.

The preparation of the inducer applied liquid: Dilute the inducer reserve fluid for 10 times, for example,

add 10 mL of the reagent reserve to 90 mL of distilled water and mix thoroughly.

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Unit:

Price: $55

Product PDFs

Datasheet:

NR (EC 1.7.1.3) is a key enzyme in the transformation of plant nitrate nitrogen into ammonia nitrogen as

well as an induction enzyme, which widely exists in plants and has an impact on crop yield and quality.

NR catalyzed nitrate reduction to nitrite, with NO₃ˉ + NADH + H^＋→ NO₂ˉ + NAD^＋+ H₂O. NADH has a

characteristic absorption peak at 340 nm. The change of absorbance at 340 nm can indicate the enzyme

activity.

Reagents and Equipment Required but Not Provided.

Table centrifuge, pipettes, spectrophotometer, water-bath, 1 mL quartz cuvette, mortar/homogeniser, ice

and distilled water.

Protocol

I. Sample Preparation

1. Put proper inducers in a beaker, wash fresh specimens and then drain with filter paper. Put the

specimens in the inducer applied liquid(covered), protected from light, immerse 2 hours. Take out the

samples and drain with filter paper. Frozen at -20℃ for 30 minutes, then take out the sample, drain with

filter paper. (Conduct induction treatment as needed)

2. Put 0.1 g of induced sample into 1 mL of Extraction solution and fully grinding on ice. Centrifuge at

4000×g for 10 minutes at 4℃ to remove insoluble materials, and take the supernatant on ice for testing.

II. Determination

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