NR (EC 1.7.1.3) is a key enzyme in the transformation of plant nitrate nitrogen into ammonia nitrogen as
well as an induction enzyme, widely exists in plants and has an impact on crop yield and quality.
NR catalyzed nitrate reduction to nitrite, with NO3ˉ + NADH + H+→ NO2ˉ + NAD++ H2O. NADH has a
characteristic absorption peak at 340 nm. The change of absorbance at 340 nm can indicate the enzyme
activity.
Reagents and Equipment Required but Not Provided.
Table centrifuge, water-bath, micro quartz cuvette/96 well UV flat-bottom plate, spectrophotometer/
microplate reader, mortar/ homogenate, ice and distilled water.