Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The microbial
quantity of soil, organic matter content, total nitrogen and available nitrogen content have positive
correlation with soil urease activity. Soil nitrogen status is determined by soil urease activity.
The ammonia is determined by the indophenol blue method, resulting in blue indophenol produced is
proportional to the concentration of ammonia.
Reagents and Equipment Required but Not Provided.
Spectrophotometer, thermostat water bath, transferpettor, 1 mL glass cuvette, ice, 30-50 mesh sieve (or
smaller), distilled water, Methylbenzene (Express delivery not allowed), distilled water.
