Urease is an enzyme that catalyzes the hydrolysis of urea into carbon dioxide and ammonia. The microbial
quantity of soil, organic matter content, total nitrogen and available nitrogen content have positive
correlation with soil urease activity. Soil nitrogen status is determined by soil urease activity. The
ammonia is determined by the indophenol blue method, resulting in blue indophenol produced is
proportional to the concentration of ammonia.
Reagents and Equipment Required but Not Provided.
Spectrophotometer/microplate reader, thermostat water bath, transferpettor, micro glass cuvette/ 96 well
flat-bottom plate, ice, 30-50 mesh sieve (or smaller), distilled water, Methylbenzene (Express delivery not
allowed) and distilled water.