Soil β-glucosidase (S-β-GC) can catalyze the hydrolysis of glycoside bonds between aryl or hydroxyl
groups and glycosylated atomic groups to generate glucose. It is an important component of cellulose
decomposition enzyme system and has important physiological functions in the carbohydrate metabolism
of soil microorganisms.
S-β-GC can catalyze the p-nitrophenyl-β-D-glucopyranoside to p-nitrophenol. The product is slightly
yellow and has characteristic of absorption at 400 nm.
Reagents and Equipment Required but Not Provided
Table centrifuge, water-bath, transferpettor, spectrophotometer, 1 mL glass cuvette, ice, 30 mesh sieve
(or smaller), methylbenzene (express delivery is not allowed) and distilled water.
Preparation
I. Sample processing:
Air dry the fresh soil sample naturally or in an oven at 37℃, and sieve it through 30~50 meshes.
II. Determination