Soil β-glucosidase(S-β- GC) Activity Assay Kit

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土壤β-葡萄糖苷酶(S-β-GC)活性检测试剂盒

Cat:

NA0616

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Reagent I: Methylbenzene, liquid 5 mL×1. Storage at 4℃. Requird but not provided.

Reagent Ⅱ: Powder×1. Storage at -20℃. Add 13 mL of distilled water to per bottle before use, and

dissolve it fully. The left reagent store at 20℃.

Reagent Ⅲ: Liquid 30 mL×1. Storage at 4℃.

Reagent IⅤ: Liquid 20 mL×1. Storage at 4℃.

Standard: Liquid × 1, 5 mmol/L p-nitrophenol solution. Take 100 μL of standard solution and add it to

400 μL of Reagent Ⅲ to get 1 mmol/L standard solution. Dilute the solution for 10 times to 100 μmol/L.

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Unit:

Price: $257

Product PDFs

Datasheet:

Soil β-glucosidase (S-β-GC) can catalyze the hydrolysis of glycoside bonds between aryl or hydroxyl

groups and glycosylated atomic groups to generate glucose. It is an important component of cellulose

decomposition enzyme system and has important physiological functions in the carbohydrate metabolism

of soil microorganisms.

S-β-GC can catalyze the p-nitrophenyl-β-D-glucopyranoside to p-nitrophenol. The product is slightly

yellow and has characteristic of absorption at 400 nm.

Reagents and Equipment Required but Not Provided.

Table centrifuge, water-bath, transferpettor, spectrophotometer/microplate reader, 30 mesh sieve (or

smaller), micro cuvette/96 well flat-bottom plate, methylbenzene (express delivery is not allowed), ice and

distilled water.

Preparation

I. Sample processing:

Air dry the fresh soil sample naturally or in an oven at 37℃, and sieve it through 30~50 meshes.

II. Determination

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