Phenylalanine Ammonia-lyase (PAL) is widely found in various plants and a few microorganisms. It is a
key enzyme in phenylpropanoid metabolism. PAL is closely related to some important secondary
substances synthetic such as lignin, isoflavones phytoalexin, flavonoid pigments, and play an important
role in normal growth and development in plants and against the bacteria resist.
L-phenylalanine can be decomposed into trans-cinnamic acid and ammonia by PAL, and trans-cinnamic
acid has the maximum absorption value at 290 nm. In this kit, the activity of PAL can be calculated by
measuring the absorbance increased rate.
Reagents and Equipments Required but Not Provided:
Spectrophotometer, water bath, refrigerated centrifuge, transferpettor, 1 mL quartz cuvette, mortar
/homogenizer, ice and distilled water.
