Trehalose is found in a large number of organisms, including bacteria, algae, yeast, plants, insects, and
other invertebrates. As trehalose has unique biological characteristics different from other carbohydrates, it
can protect organisms' cell proteins, fats, sugars, nucleic acids and other groups in harsh environments such
as drought, high temperature, dehydration, freezing, high osmotic pressure and toxic substances points are
not impaired.
The measurement method is anthrone colorimetric method. It has the advantages of high sensitivity,
simple and fast, and suitable for the determination of trace samples. However, the anthrone colorimetric
method also has certain defects. If the sample contains soluble sugar, it will affect the determination. This
kit is recommended for determination of samples that do not contain soluble sugar other than trehalose.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, water bath, adjustable transferpettor, mortar/homogenizer, centrifuge, ice, 1 mL glass
cuvette, concentrated sulfuric (H2SO4) acid and distilled water.
