Sucrase (EC 3.2.1.26) is one of the key enzymes in carbohydrate digestion and absorption. It can
hydrolyzes sucrose to produce corresponding monosaccharides which are absorbed by the body.
3.5-Dinitrosalicylic acid is reduced to brown-red amino compound by co-heating with reducing sugar. The
absorbance ratio of brown-red amino compound is in direct proportion to the contents of reducing sugar.
This product uses the 3.5-dinitrosalicylic acid method to determine the content of reducing sugars
produced by plant sucrase catalyzing sucrose degradation, then the hydrolysis rate of plant sucrase can be
obtained.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, water bath, refrigerated centrifuge, adjustable transferpettor, 1 mL glass cuvette,
mortar/homogenizer, ice and distilled water.
