Catalase(CAT) Activity Assay Kit

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过氧化氢酶(CAT)活性检测试剂盒

Cat:

NA0386

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/96S

Storage instructions：

2-8℃

Product Overview：

Components:

Extraction reagent: Liquid 110 mL×1. Storage at 4℃；

Reagent I: Liquid 30 mL×1. Storage at 4℃.

Reagent Ⅱ: Liquid 110 μL×1. Storage at 4℃. Centrifuge before use.

CAT working reagent: Add 25 µL of Reagent Ⅱ to 5 mL of Reagent I before use, mix thoroughly as

Working solution (about 26T). Or according to the proportion of preparation, the reagent should be

prepared just before use.

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Unit:

Price: $35

Product PDFs

Datasheet:

CAT is an enzyme found broadly in animals, plants, microorganisms and cultured cells. It is the main

enzyme of clearing H₂O₂, which plays an important role in the active oxygen scavenging system.

H₂O₂has characteristic absorption peak at 240 nm. It can be decomposed into water and oxygen by CAT

which makes the absorbance of reagent at 240 nm decreases. The activity of CAT can be calculated

according to the change rate of absorbance.

Reagents and Equipment Required but Not Provided:

Spectrophotometer/microplate reader, refrigerated centrifuge, transferpettor, micro quartz cuvette/96 well

UV flat-bottom plate, mortar/ homogenizer, ice and distilled water.

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