Trehalose is found in a large number of organisms, including bacteria, algae, yeast, plants, insects, and
other invertebrates. Because trehalose has unique biological characteristics different from other
carbohydrates, it can protect organisms' cell proteins, fats, sugars, nucleic acids and other groups in harsh
environments such as drought, high temperature, dehydration, freezing, high osmotic pressure and toxic
substances Points are not impaired.
The measurement method is anthrone colorimetric method. It has the advantages of high sensitivity, simple
and fast, and suitable for the determination of trace samples. However, the anthrone colorimetric method
also has certain defects. If the sample contains soluble sugar, it will affect the determination. This kit is
recommended for determination of samples that do not contain soluble sugar other than trehalose.
Reagents and Equipments Required but Not Provided:
Spectrophotometer/Microplate reader, water bath, adjustable transferpettor, mortar/homogenizer,
centrifuge, micro glass cuvette/96-well plate, concentrated sulfuric (H2SO4) acid and distilled water.
