β-1,3-glucanase(β-1,3-GA) Activity Assay Kit

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活性检测试剂盒

Cat:

NA0838

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/24S

Storage instructions：

2-8℃

Product Overview：

Components:

Extract Solution: Liquid 50 mL×1. Storage at 4℃.

Reagent I: Powder×1. Storage at 4℃. Dissolve with 3 mL of distilled water before use.

Reagent Ⅱ: Liquid 42 mL×1. Storage at 4℃.

Standard: Powder×1. Storage at 4℃. Containing 10 mg of anhydrous glucose (dry weight loss < 0.2%).

Dissolve the standard with 1 mL of distilled water to generate a 10 mg/mL glucose standard solution, store

at 4℃ and use within one week.

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Unit:

Price: $119

Product PDFs

Datasheet:

β-1,3-GA (EC 3.2.1.73) mainly exists in plants and catalyzes the hydrolysis of β-1, 3-glucoside bond. A

large number of β-1,3-GA can be induced by plant infection or other adverse conditions. Therefore, β-1,3-

GA activity assay has been widely used in plant pathology and stress physiology studies.

β-1,3-GA hydrolyzes laminarin and inner cuts β-1, 3-glucoside bond to produce reducing terminus. The

enzyme activity is calculated by measuring the rate of reducing sugar production.

Reagents and Equipment Required but Not Provided.

Spectrophotometer, desk centrifuge, adjustable pipette, 1 mL glass cuvette, mortar, ice and distilled water.

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