3-GA) Activity Assay Kit | Sunlong Medical

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葡聚糖酶

Cat:

NA0596

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

2-8℃

Product Overview：

Components:

Extraction Solution: Liquid 100 mL×1. Storage at 4℃.

Reagent I: Powder×1. Storage at 4℃. Dissolve with 3.5 mL of distilled water before use.

Reagent Ⅱ: Liquid 30 mL×1. Storage at 4℃.

Standard: Powder×1. Storage at 4℃. Containing 10 mg of anhydrous glucose (dry weight loss < 0.2%).

Dissolve the standard with 1 mL of distilled water to generate a 10 mg/mL glucose standard solution, store

at 4℃ and use within one week.

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Unit:

Price: $257

Product PDFs

Datasheet:

β-1,3-GA (EC 3.2.1.73) mainly exists in plants and catalyzes the hydrolysis of β-1, 3-glucoside bond. A

large number of β-1,3-GA can be induced by plant infection or other adverse conditions. Therefore, β-1,3-

GA activity assay has been widely used in plant pathology and stress physiology studies.

β-1,3-GA hydrolyzes laminarin and inner cuts β-1, 3-glucoside bond to produce reducing terminus. The

enzyme activity is calculated by measuring the rate of reducing sugar production.

Reagents and Equipment Required but Not Provided.

Spectrophotometer/microplate reader, desk centrifuge, adjustable pipette, micro glass cuvette/96 well flat-

bottom plate, mortar/homogenizer, illed water.

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