Sucrose Phosphorylase（SP）Activity Assay Kit

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蔗糖磷酸化酶（SP）活性检测试剂盒

Cat:

NA0831

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/24S

Storage instructions：

-20℃

Product Overview：

Components:

Reagent

Extract solution

Reagent I

Size

Storage

4℃

60 mL×1

20 mL×1

Powder×1

2mL×1

4℃

Reagent II

4℃

Reagent III

Reagent IV

Reagent V

4℃

Powder×2

Powder×1

Powder×3

Powder×4

-20℃

Reagent VI

Reagent VII

Solution preparation:

1. Reagent II: Dissolved with 15 mL of distilled water before use. Mix thoroughly. It can be stored for 4

weeks at 4℃.

2. Reagent IV： Dissolved with 1.5 mL of distilled water before use. Mix thoroughly. It can be stored for

2 weeks at -20℃.

3. Reagent V：Dissolved with 10 mL of distilled water before use. Mix thoroughly. It can be stored for 4

weeks at -20℃.

4. Reagent VI：Dissolved with 1 mL of distilled water before use. Mix thoroughly. It can be divided into

small tubules and preserved at -20℃. Avoid repeating freeze/thaw cycles. It can be stored for 2 weeks at -

20℃. Before use, dilute the reagent VI according to the ratio of reagent VI: distilled water=1:1.

5. Reagent VII：Dissolved with 0.7 mL of distilled water before use. Mix thoroughly. It can be divided

into small tubules and preserved at -20℃. It can be stored for 2 weeks at -20℃. Before use, dilute the reagent

VII according to the ratio of reagent VII: distilled water=1:1.

6. All reagents stored at -20℃ can be stored in aliquots to avoid repeated freezing and thawing

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Unit:

Price: $528

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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Sucrose Phosphorylase (SP) (EC2.4.1.7) mainly exists in microorganisms and plants. Sucrose

phosphorylase cleaves the glucosidic bond and catalyzes the transfer of the glucosyl group to fructose, xylose,

galactose and rhamnose to synthesize the corresponding glucosyl oligosaccharides. In addition, sucrose

phosphorylase can also catalyze the synthesis of arbutin from hydroquinone, which has a strong whitening

effect and has important applications in the cosmetics industry.

BC0450 -- page 1 / 4

Sucrose phosphorylase can use phosphoric acid as the receptor to catalyze the production of glucose 1-

phosphate from sucrose, which is transformed into glucose 6-phosphate under the catalysis of glucose

phosphate mutase, and reduces NADP+ to NADPH under the action of glucose 6-phosphate dehydrogenase.

This results in an increase in light absorption at 340nm. The sucrose phosphorylase activity can be calculated

by measuring the increase rate of absorbance at 340nm.

Reagents and Equipment Required but Not Provided：

Ultraviolet spectrophotometer, low temperature centrifuge, constant temperature incubator, adjustable

pipette, mortar/homogenizer, 1 mL quartz cuvette, ice and distilled water.

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