Pyruvate Kinase(PK) Activity Assay Kit

Home > Product > Test Kit > Pyruvate Kinase(PK) Activity Assay Kit

丙酮酸激酶(PK)活性检测试剂盒

Cat:

NA0826

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: 60 mL ×1, Storage at 4℃.

Solution I: 45 mL×1, Storage at 4℃.

Solution II: Powder×1. Storage at -20℃.

Solution III: Powder×1. Storage at -20℃. Add 1.5 mL of distilled water to each tube and dissolve fully

when the solution will be used. The left reagent can still store at 4℃ for one week.

Solution IV: Liquid 50 μL×1. Storage at 4℃. The liquid is placed in the EP tube in the reagent bottle.

Before use, according to the amount of volume ratio Solution III: distilled water=1:20, mix well, place on

ice for standby, prepare when the solution will be used;

prev next

Unit:

Price: $103

Product PDFs

Datasheet:

Pyruvate Kinase (PK, EC 2.7.1.40) is widely exists in animals, plants, microorganisms and cultured cells.

It could catalyze the final step of the glycolysis process. PK is one of the major rate-limiting enzymes in

the glycolysis process and one of the key enzymes for ATP production. Therefore, the determination of PK

activity is of great significance.

PK catalyzes the generation of ATP and pyruvate from phosphoenolpyruvate and ADP. Lactate

dehydrogenase further catalyzed NADH and pyruvate to generate lactic acid and NAD⁺. The NADH

degradation rate can measured at 340 nm to reflect the activity of PK.

Reagents and Equipment Required but Not Provided

Spectrophotometer, table centrifuge, water-bath, adjustable pipette, 1 mL quartz cuvette, mortar, ice and

distilled water.

Product Feedback Wall