Pyruvate Kinase (PK, EC 2.7.1.40) is widely exists in animals, plants, microorganisms and cultured
cells. It could catalyze the final step of the glycolysis process. PK is one of the major rate-limiting enzymes
in the glycolysis process and one of the key enzymes for ATP production. Therefore, the determination of
PK activity is of great significance.
PK catalyzes the generation of ATP and pyruvate from phosphoenolpyruvate and ADP. Lactate
dehydrogenase further catalyze NADH and pyruvate to generate lactic acid and NAD+. The NADH
degradation rate can measured at 340 nm to reflect the activity of PK.
Reagents and Equipment Required but Not Provided
Spectrophotometer/microplate reader, table centrifuge, water-bath, adjustable pipette, micro quartz
cuvette/96 well flat-bottom plate (UV plate), ice, mortar/homogenizer and distilled water.
