Sucrose is the main form of transport of photosynthetic products from source (leaf, etc.) to "sink" organs.
Sucrose synthetase (SS, EC 2.4.1.13) catalyzes the synthesis of sucrose from free fructose and glucose in
plants.
SS catalyzes the reaction between free fructose and glucose donor UDPG to generate sucrose, and the
reaction between sucrose and resorcinol can show color changes. There is a characteristic absorption peak
at 480 nm, and the enzyme activity of SS is proportional to the color.
Reagents and Equipment Required but Not Provided
Spectrophotometer, water-bath, table centrifuge, adjustable pipette, 1 mL glass cuvette, ice,
mortar/homogenizer and distilled water.