Chlorophyll is widely found in green plant tissues. It is the organelle of photosynthesis. Its content is
closely related to photosynthesis and nutrition. It is an important indicator of plant growth.
The maximum absorption of chlorophyll a and b was at 645 nm and 663 nm. According to the empirical
formula, the contents of chlorophyll a, chlorophyll b and total chlorophyll can be calculated.
Reagents and Equipment Required but Not Provided.
Spectrophotometer/ microplate reader, micro glass cuvette/96 well flat bottom plate (non polystyrene 96
well plate is recommended), transferpettor, balance, mortar/homogenizer, silver paper, distilled water,
anhydrous ethanol and acetone.
