Creatine Kinase(CK) Activity Assay Kit

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肌酸激酶（CK）活性检测试剂盒

Cat:

NA0784

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: 60 mL ×1. Storage at 4℃.

Reagent I: powder×1, stored in dark at -20℃.Dissolved in 10 mL of distilled water before use, the unused

reagentshall be stored at -20℃ after repacking, repeated freezing and thawing are prohibited.

Reagent II: powder×1, stored at -20℃.Dissolved in 0.5 mL of distilled water before use, the unused

reagent shall be stored at -20℃ after repacking, repeated freezing and thawing are prohibited.

Reagent III: powder×2, stored at -20℃.Dissolved in 0.5 mL of distilled water before use, the reagents that

cannot be used up shall be stored at -20℃ after repacking, repeated freezing and thawing are prohibited.

ReagentIV: powder×1, stored at -20℃.Dissolved in 0.65 mL of distilled water before use, the unused

reagent shall be stored at -20℃ after repacking, repeated freezing and thawing are prohibited.

Reagent V: 15 mL ×1. Storage at 4℃.

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Unit:

Price: $273

Product PDFs

Datasheet:

Creatine kinase (CK) (EC 2.7.3.2) is also known as creatine phosphokinase, which mainly exists in heart,

muscle and brain. It can reversibly catalyze the trans-phosphoryl reaction between creatine and ATP. It is

an important kinase directly related to cell energy transport, muscle contraction and ATP regeneration.

CK catalyzes creatine phosphate and ADP to generate creatine and ATP, hexokinase catalyzes ATP and

glucose to generate glucose-6-phosphate, and glucose-6-phosphate dehydrogenase catalyzes glucose-6-

phosphate and NADP⁺to generate NADPH, resulting in an increase of 340 nm light absorption value,

which is used to express CK enzyme activity.

Reagents and Equipment Required but Not Provided

Scales, low temperature centrifuge, constant temperature water bath, spectrophotometer, 1 mL quartz

cuvette and distilled water.

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