γ-glutamyltranspeptidase (γ-GT) is a key enzyme in γ-glutanyl cycle, which catalyzes the degradation of
GSH. γ-GT catalyzes the transfer of γ-glutamyl groups from GSH or other γ-glutamyl compounds to
receptors. It can also catalyze the hydrolysis of GSH andother γ-glutamyl compounds to produce
glutamate, which plays an important role in the metabolism of extracellular glutathione.
γ-GT catalyzes the transfer of γ-glutamyl in glutamyl p-nitroaniline to N-glycylglycine to form p-
nitroaniline with characteristic light absorption at 405 nm.γ-GT enzyme activity is calculated by measuring
the increase rate of light absorption at 405nm.
Reagents and Equipment Required but Not Provided
Spectrophotometer, centrifuge, water-bath,adjustable pipette, 1mL glass cuvette, ice, mortar/homogenizer
and distilled water.
