γ-glutamyltranspeptidase (γ-GT) is a key enzyme in γ-glutanyl cycle, which catalyzes the degradation of
GSH. γ-GT catalyzes the transfer of γ-glutamyl groups from GSH or other γ-glutamyl compounds to
receptors. It can also catalyze the hydrolysis of GSH and other γ-glutamyl compounds to produce
glutamate, which plays an important role in the metabolism of extracellular glutathione.
γ-GT catalyzes the transfer of γ-glutamyl in glutamyl p-nitroaniline to N-glycylglycine to form p-
nitroaniline with characteristic light absorption at 405 nm. γ-GT enzyme activity was calculated by
measuring the increase rate of light absorption at 405 nm.
Reagents and Equipment Required but Not Provided.
Spectrophotometer/Microplate reader,ultra-micro glass cuvette/96 well flat-bottom plate, low temperature
centrifuge, water-bath, adjustable pipette, ice, mortar/homogenizer and distilled water.
