The sulfhydryl group in organism mainly includes non-protein sulfhydryl group and protein sulfhydryl
group. Sulfhydryl compounds have important detoxification function in vivo. It has very important
physiological significance to the self-regulation of organism.
The thiol group reacts with 5,5 '- dithio-bis-nitrobenzoic acid (DTNB) to form a yellow compound. It has a
maximum absorption peak at 412 nm.
Reagents and Equipment Required but Not Provided.
Spectrophotometer/microplate reader, table centrifuge, water bath, adjustable pipette, micro glass
cuvette/96 well flat-bottom plate, methanol, mortar/homogenizer and distilled water.