Nitrogen is an essential element of living organisms. Ammonium nitrogen enters plant cells and forms
amino acids or amides. Ammonia nitrogen content in plant tissues can reflect the degree of plant stress.
Alpha - amino acid can react with hydrated indene triketone to forms blue-purple compound which has
characteristic at 570 nm. The amino acid content was calculated by measuring absorbance at 570 nm.
Reagents and Equipments Required but Not Provided:
Spectrophotometer/ microplate reader, micro glass cuvette/96 well flat-bottom plate, transferpettor, water
bath, desk centrifuge, mortar/ homogenizer, anhydrous ethanol, ice and distilled water.
