Pyruvate connects glucose, fatty acid and amino acid metabolism through acetyl CoA and plays an
important pivotal role.
Pyruvate reacts with 2, 4-dinitrophenylhydrazine to produce pyruvate -2, 4-dinitrophenylhydrazone, which
is fuchsia-red in alkaline solution.
Reagents and Equipment Required but Not Provided.
Table centrifuge, transferpettor, spectrophotometer/microplate reader, micro glass cuvette/96 well flat-
bottom plate, ice, mortar/homogenizer and distilled water.
