Phosphoglycerate Kinase(PGK) Activity Assay Kit

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3-磷酸甘油酸激酶（PGK）活性检测试剂盒

Cat:

NA0710

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: Liquid 60 mL×1, store at 4℃;

Reagent I: Liquid 25 mL×1, store at 4℃ and protect from light;

Reagent II: Powder×1, store at -20℃ and protect from light. Add 5 mL distilled water when the solution

will be used. Mix thoroughly. The rest of reagent store at -20℃; Do not freeze and thaw repeatedly;

Reagent III: Powder×1, store at -20℃ and protect from light. Add 2.5 mL distilled water when the solution

will be used. Mix thoroughly. The rest of reagent store at -20℃; Do not freeze and thaw repeatedly;

Reagent Ⅳ: Powder×3, store at -20℃ and protect from light. Add 1 mL distilled water when the solution

will be used. Mix thoroughly. The rest of reagent store at -20℃; Do not freeze and thaw repeatedly;

Reagent Ⅴ: Powder×1, store at -20℃ and protect from light. Add 10 mL distilled water when the solution

will be used. Mix thoroughly. The rest of reagent store at -20℃; Do not freeze and thaw repeatedly.

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Unit:

Price: $1320

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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PGK is the key enzyme of glycolysis. It's also a key enzyme for organisms to survive. It widely exists in

animals, plants and microorganisms. It has many biological functions, such as affecting DNA replication

and repair, stimulating RNA synthesis of virus, and is widely used in drug target design.

PGK catalyzes the production of 1,3-diphosphoglyceride and ADP from 3-phosphoglycerate and ATP. 1,3-

diphosphoglyceride produces 3-phosphoglyceraldehyde, NAD and phosphoric acid under the action of 3-

phosphoglyceraldehyde dehydrogenase and NADH. The absorbance decreased at 340nm. It reflects the

activity of 3-phosphoglycerate kinase.

Required but Not Provided:

Ultraviolet spectrophotometer, desk centrifuge, water-bath, transferpettor, 1 mL quartz cuvette，

mortar/homogenizer, ice and distilled water.

Protocol

I. Preparation:

1. Tissue: according to the tissue weight (g): the volume of the Extract solution (mL) is 1:5-10. It is

suggested that add 1 mL of Extract solution to 0.1 g of tissue. Homogenate on ice. Centrifuge at 10000 g

4℃ for 10 minutes. Take the supernatant on ice for test.

2. Cells: according to the number of the cells (10⁴): the volume of the Extract solution (mL) is

500~1000:1. It is suggested that add 1 mL of Extract solution to 5 million of cells. Breaking cells by

ultrasonic wave in ice bath (power 300W, ultrasonic 3s, interval 7s, total time 3 min). Centrifuge at 10000

g 4℃ for 10 minutes. Take the supernatant on ice for test.

3. Serum(plasma): detect directly.

II. Determination

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