ACP is an enzyme that catalyzes the hydrolysis of proteins in acidic environments. The enzyme is mainly
used in alcohol fermentation, beer brewing, fur softening, fruit wine clarification, soy sauce brewing, feed
and so on.
In acidic condition, ACP can catalyze the hydrolysis of casein to produce tyrosine; in alkaline condition,
tyrosine reduces phosphomolybdic acid compound to tungsten blue which has characteristic absorption
peak at 680 nm, and the activity of ACP is calculated by measuring its absorbance increase.
Required but not provided:
Mortar/homogenizer, desk centrifuge, spectrophotometer/microplate reader, water bath, micro glass
cuvette/96 well flat-bottom plate, magnetic stirrer, transferpettor, 1.5 mL centrifuge tube and distilled
water.
