NP catalyze the hydrolysis of protein under certain temperature and neutral PH conditions. It has the
characteristics of safety, non-toxicity, strong hydrolysis ability and wide range of action. So NP is often
used in the production of food, feed, cosmetics and nutritional health products.
In neutral conditions, NP can catalyze the hydrolysis of casein to produce tyrosine. In alkaline condition,
tyrosine reduces phosphomolybdic acid compounds to tungsten blue, which has a characteristic absorption
peak at 680 nm.
Required but not provided:
Mortar/homogenizer, desk centrifuge, spectrophotometer/microplate reader, micro glass cuvette/96 well
flat-bottom plate, water bath, magnetic stirrer, transferpettor, 1.5 mL centrifuge tube and distilled water.
