AKP is a serine protease, which catalyzes the hydrolysis of protein peptide bond in alkaline condition. In
addition, the enzyme can hydrolyze ester bond and amide bond. It has the function of trans ester and trans
peptide. The enzyme is one of the main industrial enzymes, which is widely used in pharmaceutical, silk,
food, leather and other industries.
In alkaline condition, AKP hydrolyzes casein to produce tyrosine. In alkaline condition, tyrosine
reduced phosphomolybdic acid to tungsten blue. Tungsten blue has a characteristic absorption peak at 680
nm. The activity of AKP can be calculated by measuring the increasing rate of 680 nm absorbance.
Required but not provided:
Mortar/homogenizer, desk centrifuge, spectrophotometer/microplate reader, micro glass cuvette/96 well
flat-bottom plate, water bath, magnetic stirrer, transferpettor, 1.5 mL centrifuge tube and distilled water.
