Trypsin is a serine protease from the PA clan superfamily, found in the digestive system of many
vertebrates, where it hydrolyses proteins. In the duodenum, trypsin catalyzes the hydrolysis of peptide
bonds, breaking down proteins into smaller peptides. The peptide products are then further hydrolyzed into
amino acids via other proteases, rendering them available for absorption into the blood stream. Trypsin is
widely used in the treatment of local edema, hematoma and abscess due to the pyothorax, hemothorax,
surgical inflammation, ulcer, traumatic injury, etc. Trypsin catalyzes and hydrolyzes the ester bonds of
BAEE, producing BA, BA has absorpotion at 253 nm. The activity of trypsin is calculated by measuring
the increase rate of 253 nm absorbance.
Reagents and Equipment Required but Not Provided:
Microplate reader/ spectrophotometer，transferpettor, micro quartz cuvette/96 well UV plate, centrifuge,
water-bath, transferpettor, mortar/ homogenizer, ice, distilled water.
