Lipoproteinlipase (LPL) is a speed reducing enzyme for the degradation of triglycerides. It can catalyzes
the hydrolysis of triglycerides to fatty acids and monoglycerides. It is mainly synthesized in liver
parenchymal cells and plays an important role in lipid metabolism and transport.
Lipoproteinlipase catalyzes the hydrolysis of 4-nitrophenylpalmitate to produce 4-nitrophenol with a
characteristic absorption peak at 400 nm.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, low temperature centrifuge, water-bath, 1 mL glass cuvette, transferpettor,
mortar/homogenizer, EP tube, ice, distilled water.
Operation
