Lipoproteinlipase (LPL) is a speed reducing enzyme for the degradation of triglycerides. It can catalyze the
hydrolysis of triglycerides to fatty acids and monoglycerides. It is mainly synthesized in liver parenchymal
cells and plays an important role in lipid metabolism and transport.
Lipoproteinlipase catalyzes the hydrolysis of 4-nitrophenylpalmitate to produce 4-nitrophenol with a
characteristic absorption peak at 400 nm.
Reagents and Equipment Required but Not Provided:
Spectrophotometer/Microplate reader, low temperature centrifuge, water-bath, micro glass cuvette/96 well
flat-bottom plate, transferpettor, mortar/homogenizer, ice, distilled water.
Operation
