Trehalase(THL, EC 3.2.1.28) is an enzyme found broadly in animals, plants, microorganisms and cultured
cells.The main function of THL is to break down trehalose to produce glucose and directly use it for the
energy supply of organism.
THL converts soluble trehalose to reducing sugar. 3.5-Dinitrosalicylic acid is reduced to brown-red amino
compound by co-heating with reducing sugar. The brown-red amino compound has a maximum
absorption peak at 540 nm and the absorbance ratio is in direct proportion to the contents of reducing
sugar. In this kit, the THL activity is quantified by measuring the color development at 540 nm.
Reagents and Equipments Required but Not Provided:
Spectrophotometer/microplate reader, water bath, desk centrifuge, adjustable transferpettor, micro glass
cuvette/96 well flat-bottom plate, mortar/homogenizer, ice and distilled water.