Sorbitol is widely found in animals, plants, microorganisms and cultured cells. It's a form of sugar
transport, and also closely related to biological resistance and food flavor. Therefore, it is often necessary
to detect the changes of sorbitol content in sugar metabolism, stress resistance and food research.
Sorbitol can form blue complex with copper ions in alkaline solution, which has characteristic absorption
peaks at 655 nm.
Reagents and Equipment Required but Not Provided.
Spectrophotometer, desk centrifuge, transferpettor, 1 mL glass cuvette, mortar/homogenizer, ice and
distilled water.
