Sorbitol Dehydrogenase(SDH) Activity Assay Kit

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山梨醇脱氢酶（SDH）活性检测试剂盒

Cat:

NA0689

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution: 60 mL×1. Storage at 4℃.

Reagent I: Powder×1. Storage at 4℃. Add 50 mL of Reagent Ⅴ into Reagent Ⅰ powder, dissolve and store

at 4℃.

Reagent II: Powder×1. Storage at -20℃. Before use, add 12.5 mL of Reagent Ⅴ, after fully dissolved, 2.5

mL of per bottle can be sub packed and stored at -20℃;

Reagent III: Powder×1. Storage at -20℃. Before use, add 12.5 mL of Reagent Ⅴ, after fully dissolved, 2.5

mL of per bottle can be sub packed and stored at -20℃;

Reagent IV: Powder×5. Storage at -20℃. Add 10 mL of Reagent Ⅰ before use, which is the working

solution. Prepared when the solution will be used, and it is deteriorated in 24 hours.

Reagent V: 80 mL×1. Storage at 4℃.

Standard: Powder×1. Storage at -20℃. Add 1.4 mL of distilled water before use, which is 10 μmol/mL

NADH standard.

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Unit:

Price: $174

Product PDFs

Datasheet:

SDH (EC 1.1.1.14) catalyzes the dehydrogenation of sorbitol to fructose, which is one of the key enzymes

to regulate the content of sorbitol in vivo.

SDH catalyzes the dehydrogenation of sorbitol to fructose, and the reduction of NAD⁺to NADH. The

generated NADH can transfer electrons to NBT to generate purple hairpin. According to this principle,

SDH activity can be calculated.

Reagents and Equipment Required but Not Provided:

Spectrophotometer, desk centrifuge, water bath, transferpettor, 1 mL glass cuvette, mortar/homogenizer,

ice, distilled water.

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