SDH (EC 1.1.1.14) catalyzes the dehydrogenation of sorbitol to fructose, which is one of the key enzymes
to regulate the content of sorbitol in vivo.
SDH catalyzes the dehydrogenation of sorbitol to fructose, and the reduction of NAD+ to NADH. The
generated NADH can transfer electrons to NBT to generate purple hairpin. According to this principle,
SDH activity can be calculated.
Reagents and Equipment Required but Not Provided:
Spectrophotometer/Microplate reader, desk centrifuge, water bath, transferpettor, micro glass cuvette/96
well flat-bottom plate, mortar/homogenizer, ice, distilled water.