GCDH (EC 1.1.1.47) catalyzes D-glucose and NAD(P) to form D-Gluconic acid and NAD(P)H, which are
mainly found in the liver of many microorganisms and higher animals. GCDH is an ideal enzyme for the
preparation of high content fructooligosaccharide, and it is also a diagnostic enzyme for clinical blood
glucose determination, which can be widely used in food industry and pharmaceutical industry.
GCDH catalyzes the formation of d-Gluconic acid and NADH from D-glucose and NAD. The activity of
glucose dehydrogenase can be reflected by the change of absorbance value of NADH at 340 nm.
Required material
Spectrophotometer, tabletop centrifuge, water bath, 1 mL quartz cuvette, transferpettor, mortar/
homogenizer, ice and distilled water.