Soil Nitrite Reductase（S-NiR）Activity Assay Kit

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土壤亚硝酸还原酶（S-NiR）活性检测试剂盒

Cat:

NA0653

Assay Type:

Spectrophotometer

Brand:

sunlong medical

Specificity:

50T/24S

Storage instructions：

2-8℃

Product Overview：

Components:

Reagent 1: Powder×1, storage at 4℃. Dissolve with 1mL of distilled water before use. The reagent can be

saved for 2 weeks at 4℃. Dilute 400 times with distilled water before use.

Reagent 2: Powder×1, storage at 4℃. Dissolve with 15mL of distilled water before use. The reagent can be

saved for 2 weeks at 4℃.

Reagent 3:15 mL×1, storage at 4℃. This solution is a saturated solution, just use the supernatant

Reagent 4: 25 mL×1, storage at RT and protected from light.

Reagent 5: 25 mL×1, storage at RT and protected from light.

Standard: 1 mL×1, storage at 4℃. 10 μmol/mL of NaNO₂standard solution.

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Unit:

Price: $165

Product PDFs

Datasheet:

Soil nitrite reductase (S-NiR) is one of the key enzymes in denitrification. It is a reductase produced by soil

denitrifying bacteria. It can reduce NO₂to NO. The activity reflects the conversion efficiency of nitrogen

in the process of biodegradation, and provides a certain basis for the study of nitrogen conversion.

Nitrite reductase can reduce NO₂to NO, and reduce the NO₂in the sample to participate in the

diazotization reaction to produce a purple-red compound, that is, the change in absorbance at 540nm can

reflect the activity of nitrite reductase in soil.

Reagents and Equipment Required but Not Provided:

Spectrophotometer, adjustable transferpettor, balance, mortar/homogenizer, centrifuge, 1mL glass cuvette,

sieve (30-50 mesh, or smaller), ice and distilled water.

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