Phytase (Phytase), is a binding enzyme of protein and sugar. Phytase can decompose phytic acid to
produce inorganic phosphorus and inositol, which greatly improves the utilization rate of nutrients by
organisms. Phytase widely exists in plants, animal tissues and microorganisms. Now, microorganisms are
used to synthesize phytase for production and application. Phytase has extensive research value in the
fields of food production and animal husbandry.
Under certain environmental conditions, phytase can decompose sodium phytate to generate inorganic
phosphorus and inositol derivatives. Under acidic conditions, inorganic phosphorus and ammonium
molybdate chromogen react. A blue molybdenum blue substance is produced, which has a characteristic
absorption peak at 700 nm, and the activity of phytase can be calculated by measuring the content of
inorganic phosphorus.
Reagents and Equipment Required but Not Provided.
Spectrophotometer, Table Centrifuge, Water-bath/Constant Temperature Incubator, 1 mL Glass
Cuvette, Mortar/Homogenate/Cell ultrasonic Crusher, Ultrasonic Dissolver, Gyrotron Oscillator, Ice and
Distilled Water, Concentrated sulfuric acid.
