Formaldehyde dehydrogenase exists in most prokaryotes and all eukaryotes. It is an oxidoreductase that
converts formaldehyde. Formaldehyde dehydrogenase can catalyze formaldehyde and NAD+ to produce
NADH. The absorbance at 340 nm will increase. By measuring the change at 340nm, the activity of
formaldehyde dehydrogenase can be calculated.
Reagents and Equipment Required but Not Provided:
Ultraviolet spectrophotometer, desk centrifuge, pipette, 1 mL quartz cuvette, mortar/homogenizer, ice and
distilled water.