Tartrate Resistant Acid Phosphatase（TRAP）Activity Assay Kit

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抗酒石酸酸性磷酸酶（TRAP）活性检测试剂盒

Cat:

NA0136

Assay Type:

Spectrophotometer/Microplate Reader

Brand:

sunlong medical

Specificity:

100T/48S

Storage instructions：

-20℃

Product Overview：

Components:

Extract solution:60 mL×1. Storage at 2-8℃.

Reagent I: 4 mL×1. Storage at 2-8℃.

Reagent II: powder×1,. Storage at 2-8℃. Before use, add 1.2mL of reagent I, dissolve it fully. If the

reagent does not dissolve sufficiently, heat the reagent to 50℃ to promote its dissolution. Unused reagents

can be stored at 2-8℃ for 8 weeks.

Reagent III: powder×2,. Storage at -20℃. Before use, add 1mL of water, dissolve it fully. Unused

reagents can be stored at -20℃ for 4 weeks.Avoid repeated freezing and thawing. One reagent can do

100T after dissolving. To prolong the use of the kit, therefore, one more powder is given.

Reagent IV: powder×1. Storage at 2-8℃. Before use, add 5.5mL of water, dissolve it fully. Unused

reagents can be stored at 2-8℃ for 4 weeks.

Reagent V : 0.3 mL×1. Storage at 2-8℃. According to the ratio of reagent V: distilled water = 1:9

according to the number of samples before use.

Reagent VI: 1.2 mL×1. Storage at 2-8℃.

Reagent VII: 1.2 mL×1. Storage at 2-8℃.

Reagent VIII: 15 mL×1. Storage at 2-8℃.

Standard: 1 mL×1. Storage at 2-8℃. 5 μmol/mL phenol standard solution. Before use, take 100 μL

of 5 μmol/mL phenol standard solution in an EP tube, add 300 μL of distilled water and mix thoroughly to

make 1.25 μmol/mL phenol standard solution..

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Unit:

Price: $86

Product PDFs

Datasheet:

Other Information
Citations
Protein Attributes
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Tartrate resistant acid phosphatase (TRAP) is a characteristic enzyme of osteoclasts. TRAP is

involved in the degradation of solid calcium and phosphorus mineralized substrates in the bone matrix, and

its expression and secretion are closely related to osteoclast function.

In the presence of tartaric acid, the activity of anti-tartaric acid phosphatase is not inhibited, whereas

the activity of other acid phosphatases is inhibited. Under acidic conditions, antitartaric acid phosphatase

catalyzes the production of p-nitrophenol from PNPP. The p-nitrophenol appears yellow under alkaline

conditions and can be detected by absorbance at 400 nm. The darker the yellow color of the product, the

higher the anti-tartaric acid phosphatase activity, and vice versa, the lower the enzyme activity.

Reagents and Equipment Required but Not Provided:

Spectrophotometer/microplate reader, centrifuge, water bath/incubator, adjustable pipette, Micro glass

cuvettes/96 well plates, mortar/homogenizer/cell sonicator, ice and distilled water

BC5405 – Page 1 / 4

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