Iron is one of the essential trace elements in human body. Fe2+ is the main component of hemoglobin,
myoglobin, cytochrome and other enzyme systems, which could assist in the transport of oxygen and
promote fat oxidation. Iron deficiency can easily cause anemia, metabolic disorders, and affect the immune
function of the body.
Fe2+ could react with Tripyridyltriazine to form a kind of blue compound under acid condition, which
has an absorption peak at 593 nm. Changes of the absorbance at 593nm could be measured to reflect
ferrous ion content.
Reagents and Equipment Required but Not Provided:
Spectrophotometer, balance, low temperature table centrifuge, constant temperature incubator/water
bath, 1mL glass cuvette, pipette, mortar/homogenizer/cell ultrasonic crusher, ice, distilled water and
concentrated sulfuric acid.
