Home > Product > Test Kit > Fluoride Resistant Acid Phosphatase Activity Assay Kit
抗氟离子酸性磷酸酶(FRAP)活性检测试剂盒
Cat:
NA0132
Assay Type:
Spectrophotometer/Microplate Reader
Brand:
sunlong medical
Specificity:
100T/48S
Storage instructions:
-20℃
Product Overview:
Components:  
Extract solution:60 mL×1. Storage at 2-8℃.  
Reagent I: 1.5 mL×1. Storage at 2-8℃.  
Reagent II: 1.2 mL×1. Storage at 2-8℃.  
Reagent III: powder×2,. Storage at -20℃. Before use, add 1mL of water, dissolve it fully. Unused  
reagents can be stored at -20℃ for 4 weeks.Avoid repeated freezing and thawing. One reagent can do  
100T after dissolving. To prolong the use of the kit, therefore, one more powder is given.  
Reagent IV: powder×1. Storage at 2-8℃. Before use, add 5.5mL of water, dissolve it fully. Unused  
reagents can be stored at 2-8℃ for 4 weeks.  
Reagent V : 0.3 mL×1. Storage at 2-8℃. According to the ratio of reagent V: distilled water = 1:9  
according to the number of samples before use.  
Reagent VI: 1.2 mL×1. Storage at 2-8℃.  
Reagent VII: 1.2 mL×1. Storage at 2-8℃.  
Reagent VIII: 15 mL×1. Storage at 2-8℃.  
Standard: 1 mL×1. Storage at 2-8℃. 5 μmol/mL phenol standard solution. Before use, take 100 μL  
of 5 μmol/mL phenol standard solution in an EP tube, add 300 μL of distilled water and mix thoroughly to  
make 1.25 μmol/mL phenol standard solution..  
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Unit:
Price: $
Product PDFs
Datasheet:


Fluoride resistant acid phosphatase (FRAP) is a type of acid phosphatase. FRAP is mainly found in  
the lysosomes of most cells, the prostate gland, brain, liver, spleen, and platelets.  
The activity of fluoride-resistant acid phosphatase is not inhibited by fluoride ions, whereas the  
activity of other acid phosphatases is inhibited by fluoride ions. Under acidic conditions, the fluoride-  
resistant ion acid phosphatase catalyzes the production of p-nitrophenol from PNPP. The p-nitrophenol  
appears yellow under alkaline conditions and can be detected at 400 nm absorbance. The darker the yellow  
color of the product, the higher the activity of the fluorine-resistant ion acid phosphatase, and vice versa,  
the lower the enzyme activity.  
Reagents and Equipment Required but Not Provided:  
Spectrophotometer/microplate reader, centrifuge, water bath/incubator, adjustable pipette, Micro glass  
cuvettes/96 well plates, mortar/homogenizer/cell sonicator, ice and distilled water  
 
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