The 6-phosphate glucose dehydrogenase (6-PGDH) and 6-phosphogluconate dehydrogenase (6-PGDH) in
the pentose phosphate pathway catalyze the synthesis of NADPH in turn, which is closely related to energy
balance, growth rate and cell viability. In addition, 6-PGDH plays an important role in stress physiology.
6-PGDH catalyzes the production of NADPH by 6-phosphogluconic acid and NADP+. NADPH has a
characteristic absorption peak at 340 nm, while NADP+ does not. In this kit, the activity of 6-PGDH is
determined by the increase rate of NADPH at 340 nm.
Reagents and Equipment Required but Not Provided:
Ultraviolet spectrophotometer, desk centrifuge, pipette, water bath, 1 mL quartz cuvette,
mortar/homogenizer, ice, distilled water.
