Home > Product > Test Kit > Rat 4-Hydroxynonenal (4-HNE) ELISA Kit
Cat:
NSL0889r
Detection Range:
1000pmol/ml-15.6pmol/ml
Sensitivity:
5pmol/ml
Assay Type:
Sandwich ELISA
Brand:
Sunlong Biotech
Specificity:
48T/96T
Species Reactivity:
Rat
Storage instructions:
2-8℃/-20℃
Product Overview:

One antibody molecule can be labeled with multiple biotin molecules, 

and one biotin molecule can bind a horseradish peroxidase labeled 

avidin, thus bringing a large amount of horseradish peroxidase bound 

to the antibody, which has higher sensitivity and signal amplification 

effect than the traditional direct horseradish peroxidase labeled 
antibody.

More
Unit:
Price: $
Product PDFs
Datasheet:


Purpose

The kit is to assay Rat 4-Hydroxynonenal (4-HNE)  levels in Rat serum, plasma, culture media or any biological fluid. 


Principle 
The Microelisa stripplate has been pre-coated with an monoclonal antibody or antigen and the detecting antibody or antigen is biotinylated in this kit. Samples and biotinylated polyclonal antibody or antigen are added to the appropriate Microelisa stripplate wells in sequence and washed out with TBS or PBS. Then HRP-Avidin is added to each Microelisa stripplate well. The TMB is used for coloration after the HRP-Avidin has been reacted thoroughly and washed out by TBS or PBS. The TMB will appear blue under peroxidase activity then turn yellow after the addition of the stop solution. The depth of color is positively correlated with the factors to be measured in the sample. The optical density (OD) is measured spectrophotometrically at a wavelength of 450 nm. The OD value is proportional to the concentration of Rat 4-Hydroxynonenal (4-HNE) . You can calculate the concentration of Rat 4-Hydroxynonenal (4-HNE)  in the samples by comparing the OD of the samples to the standard curve.

Assay range

1000pmol/ml-15.6pmol/ml
Intra-Assay: CV≤ 8%
Inter-Assay: CV≤ 12%
Recovery: 70 - 110 percent


Sensitivity

5pmol/ml

Storage and validity
1.Storage: -20℃.[Short-term should be stored at 2-8℃ ~2 weeks]
2.Validity: 12 months
3 . Production Date: On the lable of elisa box

Product Feedback Wall
Message :
Your Email :
Copyright © 2007-2018 Sunlong Medical All Rights Reserved.